The Hematology Journal

Login   |   Register

Open Journal Systems



DOI: http://dx.doi.org/10.4081/thj.2005.1.584

Plasma nitric oxide levels used as an indicator of doxorubicin-induced cardiotoxicity in rats

Jhon Guerra, Ana De Jesus, Pedro Santiago-Borrero, Angel Roman-Franco, Edwin Rodriguez, Maria J Crespo

Abstract


Introduction: The clinical efficacy of doxorubicin is severely limited by its cardiotoxicity. The currently noninvasive techniques used to detect this complication lack sensitivity to identify its early stages. Nitric oxide (NO) is a free radical that has been implicated in the etiology of doxorubicin-induced cardiotoxicity. In the present study, we investigated whether plasmatic NO levels can be used as a noninvasive and reliable biomarker of doxorubicin-induced cardiotoxicity. Materials and methods: Two groups of six spontaneously hypertensive rats (SHR) were used for the experiment. Group 1 received 1.5 mg/kg intraperitoneal (IP) doxorubicin weekly for up to 9 weeks. Group 2 (Control) received nine weekly IP injection of 0.5cm3 saline. Plasmatic NO levels and cardiac ejection fraction (EF%) were determined. Ventricular biopsies were analyzed by light microscopy according with the Billinghan score. Results: Doxorubicin treatment significantly increased plasmatic NO concentration (35.3075.63 mM versus 14.7272.66 mM in control animals, n 1⁄4 6, Po0.05). In addition, doxorubicin decreased EF by 23% approximately (from 77.0073.89 in controls, to 59.0075.61 in doxorubicin-treated animals, n 1⁄4 6, Po0.05). The mean score of histological cardiac damage was 2.3370.33 for doxorubicin-treated versus 0.0870.08 for controls, n 1⁄4 6, Po0.001. Discussion: Our results revealed a correlation between plasmatic NO levels, systolic function and histopathological myocardial damage. Therefore, it is plausible to postulate that plasmatic NO levels could be used as a biomarker for myocardial damage in doxorubicin-treated SHR, and may be a potential tool for noninvasive evaluation of doxorubicin-induced toxicity in humans.

Keywords


doxorubicin; nitric oxide; biomarker; cardiomyopathy; ejection fraction

Full Text:

PDF

Submitted: 2013-08-29 09:17:25
Published:
Search for citations in Google Scholar
Related articles: Google Scholar

Refbacks

  • There are currently no refbacks.


FOOTER

The Hematology Journal was merged with Haematologica in 2005.

 

 

 
 
© PAGEPress 2008-2013     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185